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Addgene

pCL1317
(Plasmid #217959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-N1
  • Backbone manufacturer
    Clonetech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H4
  • Species
    H. sapiens (human)
  • Mutation
    GGG insertion between V65 and F66
  • Entrez Gene
    H4C1 (a.k.a. H4-16, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV promoter primer
  • 3′ sequencing primer peGFP-N1-RP
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL1317 was a gift from Andrew Bowman (Addgene plasmid # 217959 ; http://n2t.net/addgene:217959 ; RRID:Addgene_217959)

  • For your References section:

    A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3. Pardal AJ, Bowman AJ. Elife. 2022 Sep 6;11:e81755. doi: 10.7554/eLife.81755. 10.7554/eLife.81755 PubMed 36066346
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