pGEX-4T_CIP2A_1-560_V5His
(Plasmid
#217922)
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PurposeE. coli expression of GST-tagged CIP2A_1-560 protein, with C-terminal V5 His tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
- Backbone size w/o insert (bp) 4966
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor E. coli overexpression, use BL21 cells
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCIP2A
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SpeciesH. sapiens (human)
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Entrez GeneCIP2A (a.k.a. KIAA1524, NOCIVA, p90)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- V5-His (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T_CIP2A_1-560_V5His was a gift from Jukka Westermarck (Addgene plasmid # 217922 ; http://n2t.net/addgene:217922 ; RRID:Addgene_217922)
