Skip to main content
Addgene

AIRAP 2: AIRAP(m1.9-T7)pGEX.KG
(Plasmid #21792)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX.KG
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5006
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Growth instructions
    Strain should be BL21
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AIRAP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1250
  • GenBank ID
    AF224494
  • Entrez Gene
    Zfand2a (a.k.a. Airap, AA407930, AU016206)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We DO NOT have a good record for how this plasmid was constructed.
The best guess is that Jin Liu used the m1.9Bam.1S primer and T7
to PCR the CDS + 3'UTR from m1.9pBS and ligated the BamH1-Xba1
fragment in frame with GST in pGEX.KG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AIRAP 2: AIRAP(m1.9-T7)pGEX.KG was a gift from David Ron (Addgene plasmid # 21792 ; http://n2t.net/addgene:21792 ; RRID:Addgene_21792)

  • For your References section:

    An arsenite-inducible 19S regulatory particle-associated protein adapts proteasomes to proteotoxicity. Stanhill A, Haynes CM, Zhang Y, Min G, Steele MC, Kalinina J, Martinez E, Pickart CM, Kong XP, Ron D. Mol Cell. 2006 Sep 15. 23(6):875-85. 10.1016/j.molcel.2006.07.023 PubMed 16973439
Related items: