N-Flag-IPO9_Lenti SFFV Puro WPRE
(Plasmid
#217916)
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PurposeExpress IPO9 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
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Backbone manufacturerTwist Bioscience
- Backbone size w/o insert (bp) 7500
- Total vector size (bp) 10681
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameimportin 9
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SpeciesH. sapiens (human)
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Insert Size (bp)10000
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Entrez GeneIPO9 (a.k.a. Imp9)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
N-Flag-IPO9_Lenti SFFV Puro WPRE was a gift from Benjamin Cravatt (Addgene plasmid # 217916 ; http://n2t.net/addgene:217916 ; RRID:Addgene_217916) -
For your References section:
Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes. Ogasawara D, Konrad DB, Tan ZY, Carey KL, Luo J, Won SJ, Li H, Carter TR, DeMeester KE, Njomen E, Schreiber SL, Xavier RJ, Melillo B, Cravatt BF. Cell Chem Biol. 2024 Nov 7:S2451-9456(24)00440-9. doi: 10.1016/j.chembiol.2024.10.005. 10.1016/j.chembiol.2024.10.005 PubMed 39547236