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Addgene

T40A+Q54A
(Plasmid #217913)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217913 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-30
  • Backbone size w/o insert (bp) 4667
  • Total vector size (bp) 5600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PXP T40A+Q54A
  • Species
    Synthetic
  • Insert Size (bp)
    933
  • Mutation
    Threonine at position 41 and 253 mutated to Alanine, Glutamine at position 55 and 267 mutated to Alanine, changes occur in each of the two coils in order to alter the coiled-coil interaction
  • Promoter T5
  • Tag / Fusion Protein
    • 6x His (N and C term)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T40A+Q54A was a gift from Cole DeForest (Addgene plasmid # 217913 ; http://n2t.net/addgene:217913 ; RRID:Addgene_217913)

  • For your References section:

    Genetically Encoded XTEN-based Hydrogels with Tunable Viscoelasticity and Biodegradability for Injectable Cell Therapies. Bennett JI, Boit MO, Gregorio NE, Zhang F, Kibler RD, Hoye JW, Prado O, Rapp PB, Murry CE, Stevens KR, DeForest CA. Adv Sci (Weinh). 2024 Mar 13:e2301708. doi: 10.1002/advs.202301708. 10.1002/advs.202301708 PubMed 38477407
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