A2C-∆N
(Plasmid
#217909)
-
PurposeExpresses bicone gas vesicles in E. coli
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 217909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET28a
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5374
- Total vector size (bp) 11187
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameA2C-∆N
-
Alt nameBicone GV
-
SpeciesAnabaena flos-aquae and Bacillus megaterium
-
Insert Size (bp)5813
- Promoter T7
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 terminator reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
A2C-∆N was a gift from Mikhail Shapiro (Addgene plasmid # 217909 ; http://n2t.net/addgene:217909 ; RRID:Addgene_217909) -
For your References section:
Truly Tiny Acoustic Biomolecules for Ultrasound Imaging and Therapy. Ling B, Gungoren B, Yao Y, Dutka P, Vassallo R, Nayak R, Smith CAB, Lee J, Swift MB, Shapiro MG. Adv Mater. 2024 Feb 26:e2307106. doi: 10.1002/adma.202307106. 10.1002/adma.202307106 PubMed 38409678