pES1004
(Plasmid
#21790)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 21790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRS406
- Backbone size w/o insert (bp) 4300
-
Vector typeYeast Expression
-
Selectable markersURA3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKss1-as2-trunc
-
Alt nameKss1
-
Alt nameKss1-as2
-
Alt nameKss1-as
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)1486
-
Mutationdeleted bases 1-14 from open reading frame E94A mutation 401 3' UTR / terminator included
-
GenBank IDNC_001139
-
Entrez GeneKSS1 (a.k.a. YGR040W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byDr. Eduard Serra ([email protected])
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pES1004 was a gift from Richard Yu (Addgene plasmid # 21790 ; http://n2t.net/addgene:21790 ; RRID:Addgene_21790) -
For your References section:
Negative feedback that improves information transmission in yeast signalling. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Nature. 2008 Dec 11. 456(7223):755-61. 10.1038/nature07513 PubMed 19079053