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Addgene

pES1004
(Plasmid #21790)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21790 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS406
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kss1-as2-trunc
  • Alt name
    Kss1
  • Alt name
    Kss1-as2
  • Alt name
    Kss1-as
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1486
  • Mutation
    deleted bases 1-14 from open reading frame E94A mutation 401 3' UTR / terminator included
  • GenBank ID
    NC_001139
  • Entrez Gene
    KSS1 (a.k.a. YGR040W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Eduard Serra ([email protected])

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pES1004 was a gift from Richard Yu (Addgene plasmid # 21790 ; http://n2t.net/addgene:21790 ; RRID:Addgene_21790)
  • For your References section:

    Negative feedback that improves information transmission in yeast signalling. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Nature. 2008 Dec 11. 456(7223):755-61. 10.1038/nature07513 PubMed 19079053