Skip to main content
Addgene

pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
(Plasmid #217896)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217896 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pACEBac1
  • Total vector size (bp) 6600
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PSMB5
  • Species
    H. sapiens (human)
  • Entrez Gene
    PSMB5 (a.k.a. LMPX, MB1)

Gene/Insert 2

  • Gene/Insert name
    PSMB6
  • Species
    H. sapiens (human)
  • Entrez Gene
    PSMB6 (a.k.a. DELTA, LMPY)

Gene/Insert 3

  • Gene/Insert name
    PSMB7
  • Species
    H. sapiens (human)
  • Entrez Gene
    PSMB7
  • Tag / Fusion Protein
    • 2xSTII (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII was a gift from Brenda Schulman (Addgene plasmid # 217896 ; http://n2t.net/addgene:217896 ; RRID:Addgene_217896)
  • For your References section:

    Visualizing chaperone-mediated multistep assembly of the human 20S proteasome. Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA. Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9. 10.1038/s41594-024-01268-9 PubMed 38600324