pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
(Plasmid
#217896)
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PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACEBac1
- Total vector size (bp) 6600
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namePSMB5
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SpeciesH. sapiens (human)
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Entrez GenePSMB5 (a.k.a. LMPX, MB1)
Gene/Insert 2
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Gene/Insert namePSMB6
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SpeciesH. sapiens (human)
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Entrez GenePSMB6 (a.k.a. DELTA, LMPY)
Gene/Insert 3
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Gene/Insert namePSMB7
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SpeciesH. sapiens (human)
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Entrez GenePSMB7
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Tag
/ Fusion Protein
- 2xSTII (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII was a gift from Brenda Schulman (Addgene plasmid # 217896 ; http://n2t.net/addgene:217896 ; RRID:Addgene_217896) -
For your References section:
Visualizing chaperone-mediated multistep assembly of the human 20S proteasome. Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA. Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9. 10.1038/s41594-024-01268-9 PubMed 38600324
Map uploaded by the depositor.
