pES1002
(Plasmid #21789)

• Depositing Lab: Richard Yu
• Publication: Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Backbone

• Vector backbone: pRS406
• Backbone size w/o insert (bp): 4700
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fus3-as2
• Alt name: Fus3
• Alt name: Fus3-as
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2063
• Mutation: contains 5' 1kb upstream UTR/promoter Q93A mutation in open reading frame 3' 400bp downstream UTR/terminator
• GenBank ID: NC_001134
• Entrez Gene: FUS3 (a.k.a. YBL016W, DAC2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Eduard Serra ()

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.