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pRY1001
(Plasmid #21787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACL7-YFP-A206K
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP-Ste12
  • Alt name
    CFP
  • Alt name
    Ste12
  • Species
    S. cerevisiae (budding yeast); A. victoria
  • Insert Size (bp)
    4254
  • Mutation
    contains 1000 bp UTR (Ste12 promoter) upstream of CFP-Ste12 ORF contains 3' ADH terminator from pACL7-YFP-A206K.
  • GenBank ID
    NC_001140
  • Entrez Gene
    STE12 (a.k.a. YHR084W)
  • Tag / Fusion Protein
    • CFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construction was complicated; please refer to parent article's supplemental materials for details of construction. KpnI site is not unique in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRY1001 was a gift from Richard Yu (Addgene plasmid # 21787 ; http://n2t.net/addgene:21787 ; RRID:Addgene_21787)

  • For your References section:

    Negative feedback that improves information transmission in yeast signalling. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Nature. 2008 Dec 11. 456(7223):755-61. 10.1038/nature07513 PubMed 19079053
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