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p4A8_M59I_T94M_BC
(Plasmid #217833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETconNK
  • Backbone manufacturer
    Whitehead Lab, Addgene #81169
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4A8 variant Fab
  • Species
    H. sapiens (human)
  • Mutation
    VH: M59I, VL: T94M

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4A8_M59I_T94M_BC was a gift from Timothy Whitehead (Addgene plasmid # 217833 ; http://n2t.net/addgene:217833 ; RRID:Addgene_217833)

  • For your References section:

    An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries. Petersen BM, Kirby MB, Chrispens KM, Irvin OM, Strawn IK, Haas CM, Walker AM, Baumer ZT, Ulmer SA, Ayala E, Rhodes ER, Guthmiller JJ, Steiner PJ, Whitehead TA. Nat Commun. 2024 May 10;15(1):3974. doi: 10.1038/s41467-024-48072-z. 10.1038/s41467-024-48072-z PubMed 38730230
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