pBDP
(Plasmid
#217827)
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PurposeStuffer plasmid with golden gate compatible bidirectional promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217827 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK084
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Backbone manufacturerJohn Dueber, Addgene #65191
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Vector typeCloning Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal1/Gal10 Bidirectional Promoter
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBDP was a gift from Timothy Whitehead (Addgene plasmid # 217827 ; http://n2t.net/addgene:217827 ; RRID:Addgene_217827) -
For your References section:
An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries. Petersen BM, Kirby MB, Chrispens KM, Irvin OM, Strawn IK, Haas CM, Walker AM, Baumer ZT, Ulmer SA, Ayala E, Rhodes ER, Guthmiller JJ, Steiner PJ, Whitehead TA. Nat Commun. 2024 May 10;15(1):3974. doi: 10.1038/s41467-024-48072-z. 10.1038/s41467-024-48072-z PubMed 38730230