pKM 1995
(Plasmid #217826)

• Purpose: Expresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.
• Depositing Lab: Aziz Sancar
• Publication: Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9. doi: 10.1021/bi00020a037.

Backbone

• Vector backbone: pMal-c2
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 6096
• Total vector size (bp): 7650
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HY4
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1554
• Entrez Gene: CRY1 (a.k.a. AT4G08920, ATCRY1, BLU1, BLUE LIGHT UNINHIBITED 1, CRYPTOCHROME 1 APOPROTEIN (BLUE LIGHT PHOTORECEPTOR, ELONGATED HYPOCOTYL 4, HY4, OOP2, OUT OF PHASE 2, T3H13.14, T3H13_14, cryptochrome 1)
• Tag / Fusion Protein:
  • Maltose-binding protein (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Pst1 (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HY4 gene was provided from Ronald Davis at Stanford University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 512 bp deletion downstream of AmpR and an I3T mutation in MBP was found.