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Addgene

pKM 1995
(Plasmid #217826)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMal-c2
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6096
  • Total vector size (bp) 7650
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HY4
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1554
  • Entrez Gene
    CRY1 (a.k.a. AT4G08920, ATCRY1, BLU1, BLUE LIGHT UNINHIBITED 1, CRYPTOCHROME 1 APOPROTEIN (BLUE LIGHT PHOTORECEPTOR, ELONGATED HYPOCOTYL 4, HY4, OOP2, OUT OF PHASE 2, T3H13.14, T3H13_14, cryptochrome 1)
  • Tag / Fusion Protein
    • Maltose-binding protein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Pst1 (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HY4 gene was provided from Ronald Davis at Stanford University.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 512 bp deletion downstream of AmpR and an I3T mutation in MBP was found.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM 1995 was a gift from Aziz Sancar (Addgene plasmid # 217826 ; http://n2t.net/addgene:217826 ; RRID:Addgene_217826)

  • For your References section:

    Putative blue-light photoreceptors from Arabidopsis thaliana and Sinapis alba with a high degree of sequence homology to DNA photolyase contain the two photolyase cofactors but lack DNA repair activity. Malhotra K, Kim ST, Batschauer A, Dawut L, Sancar A. Biochemistry. 1995 May 23;34(20):6892-9. doi: 10.1021/bi00020a037. 10.1021/bi00020a037 PubMed 7756321
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