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Addgene

pET28a-DhFld
(Plasmid #217821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone size w/o insert (bp) 429
  • Total vector size (bp) 5721
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DhFld
  • Species
    Desulfitobacterium hafniense
  • Insert Size (bp)
    429
  • Promoter T7
  • Tag / Fusion Protein
    • His6x (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-DhFld was a gift from Pamela Silver (Addgene plasmid # 217821 ; http://n2t.net/addgene:217821 ; RRID:Addgene_217821)

  • For your References section:

    Sustainable B(12)-Dependent Dehalogenation of Organohalides in E. coli. Ng TL, Silver PA. ACS Chem Biol. 2024 Feb 16;19(2):380-391. doi: 10.1021/acschembio.3c00585. Epub 2024 Jan 22. 10.1021/acschembio.3c00585 PubMed 38254247
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