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Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)
(Plasmid #217798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang, Addgene #52961
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
  • Alt name
    DRR921
  • Species
    Synthetic
  • Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
  • Promoter EF1a
  • Tag / Fusion Protein
    • BPNLS (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK394-GTGATGTCGTGTACTGGCTCC
  • 3′ sequencing primer oBK195-GGCGGTCCGGATCGACGGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921) was a gift from Benjamin Kleinstiver (Addgene plasmid # 217798 ; http://n2t.net/addgene:217798 ; RRID:Addgene_217798)

  • For your References section:

    Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases. Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, Kleinstiver BP. Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x. 10.1038/s41587-024-02324-x PubMed 39039307
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