pEGFP-PRKCZ-C20 (N-PRKCZ-C20)
(Plasmid #217762)

• Purpose: Fluorescent reporter for ceramide (putative, mammalian expression)
• Depositing Lab: Paula Nunes-Hasler
• Publication: Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. doi: 10.3390/ijms25052996.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 4989
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Protein kinase C zeta
• Alt name: PRKCZ
• Alt name: PKC2
• Species: H. sapiens (human)
• Insert Size (bp): 560
• Mutation: Linker GGSSGGGA plus C20 domain (aa 405-592)
• GenBank ID: NM_002744.6
• Entrez Gene: PRKCZ (a.k.a. PKC-ZETA, PKC2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: GGTTCAGGGGGAGGTGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA