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Addgene

pBiFC-SS-VN155(I152L)-IRE1-dLD
(Plasmid #217752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBiFC-VN155(I152L)
  • Backbone manufacturer
    pBiFC-VN155(I152L) (Addgene Plasmid #27097)
  • Backbone size w/o insert (bp) 3791
  • Total vector size (bp) 6167
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Serine/threonine-protein kinase/endoribonuclease IRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2376
  • Mutation
    deleted amino acids 29-408
  • Entrez Gene
    ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
  • Tag / Fusion Protein
    • Venus (1-154, I152L)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiFC-SS-VN155(I152L)-IRE1-dLD was a gift from Christina Chan (Addgene plasmid # 217752 ; http://n2t.net/addgene:217752 ; RRID:Addgene_217752)

  • For your References section:

    Intrinsic Structural Features of the Human IRE1alpha Transmembrane Domain Sense Membrane Lipid Saturation. Cho H, Stanzione F, Oak A, Kim GH, Yerneni S, Qi L, Sum AK, Chan C. Cell Rep. 2019 Apr 2;27(1):307-320.e5. doi: 10.1016/j.celrep.2019.03.017. 10.1016/j.celrep.2019.03.017 PubMed 30943411
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