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YIPlac204TKC-GFP-HDEL
(Plasmid #21771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YIPlac204
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Green Fluorescent Protein (GFP)
  • Alt name
    GFP
  • Alt name
    EGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    860
  • Tags / Fusion Proteins
    • preKar2 (N terminal on insert)
    • HDEL (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CTACAAAAAACACATACATAAACT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIPlac204TKC-GFP-HDEL was a gift from Benjamin Glick (Addgene plasmid # 21771 ; http://n2t.net/addgene:21771 ; RRID:Addgene_21771)

  • For your References section:

    A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae. Rossanese OW, Reinke CA, Bevis BJ, Hammond AT, Sears IB, O'Connor J, Glick BS. J Cell Biol. 2001 Apr 2. 153(1):47-62. 10.1083/jcb.153.1.47 PubMed 11285273
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