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Addgene

HA-MBP-TEVpCS-ADGRD1 CTF-FLAG
(Plasmid #217687)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFuse-hIgG1-Fc2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADGRD1
  • Alt name
    GPR133
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of residues 1-544
  • Entrez Gene
    ADGRD1 (a.k.a. GPR133, PGR25)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-MBP-TEVpCS-ADGRD1 CTF-FLAG was a gift from Stephen Blacklow (Addgene plasmid # 217687 ; http://n2t.net/addgene:217687 ; RRID:Addgene_217687)

  • For your References section:

    Heterogeneity of Tethered Agonist Signaling in Adhesion G Protein-Coupled Receptors. Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse AC, Blacklow SC. bioRxiv 2022.07.14.500097 10.1101/2022.07.14.500097
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