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pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL
(Plasmid #217676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4158
  • Total vector size (bp) 6951
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GtACR2-P2A-Voltron2ST
  • Alt name
    ORCHID (all-Optical Reporting of CHloride Ion Driving force)
  • Species
    Synthetic
  • Insert Size (bp)
    2793
  • Promoter EF‐1α
  • Tag / Fusion Protein
    • Soma targeting sequence (Kv2.1) on Voltron2 gene only. (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (unknown if destroyed)
  • 3′ cloning site Nhe1 (unknown if destroyed)
  • 5′ sequencing primer CTCCTCATGTCAATCACTCC; GTCCATGTATTCTTCGACCAG; GTGCAACATGCGGGATGATG; GTATGCGCTCTTCAGCTCTG; CAGCCACTTCATGTACTCGG
  • 3′ sequencing primer GTAATCCAGAGGTTGATTATC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Voltron2-ST gene originates from pGP-pcDNA3.1 Puro-CAG-Voltron2-ST (Addgene #172910), and was kindly provided by Eric Schreiter and Ahmed Abdelfattah. The GtACR2 gene originates from pAAV-EF1α-FRT-FLEX-GtACR2-EYFP (Addgene #114369).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL was a gift from Joseph Raimondo (Addgene plasmid # 217676 ; http://n2t.net/addgene:217676 ; RRID:Addgene_217676)

  • For your References section:

    All-optical reporting of inhibitory receptor driving force in the nervous system. Selfe JS, Steyn TJS, Shorer EF, Burman RJ, Düsterwald KM, Abdelfattah AS, Schreiter ER, Newey SE, Akerman CJ, Raimondo JV. bioRxiv 2023.08.30.555464 10.1101/2023.08.30.555464
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