PE-SUMO CRYGA
(Plasmid #217672)

• Purpose: For expression of SUMO tagged human gamma A crystallin
• Depositing Lab: Larry David
• Publication: Completion of previous SUMO tagged human crystallin submission plus plasmids for native chemical ligation to produced gamma S crystallin (unpublished)

Backbone

• Vector backbone: PE-SUMO
• Backbone manufacturer: LifeSensors
• Backbone size w/o insert (bp): 5761
• Total vector size (bp): 6257
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRYGA
• Species: H. sapiens (human)
• Insert Size (bp): 519
• GenBank ID: CRYGA
• Entrez Gene: CRYGA (a.k.a. CRY-g-A, CRYG1, CRYG5)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6XHis (N terminal on backbone)
  • Smt3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bsal (not destroyed)
• 3′ cloning site: Xbal (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Supplemental Documents:
  • pE-SUMO CRYGA.gbk
• A portion of this plasmid was derived from a plasmid made by: Origene RC224787

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses well in a soluble state. However, the SUMO tag is not removed by ULP-1 cleavage. The N-terminal methionine has been removed to mirror the form of the protein in lens that does not contain its methionine.