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PE-SUMO CRYAB
(Plasmid #217670)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217670 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PE-SUMO
  • Backbone manufacturer
    LifeSensors
  • Backbone size w/o insert (bp) 5761
  • Total vector size (bp) 6263
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRYAB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    525
  • GenBank ID
    CRYAB
  • Entrez Gene
    CRYAB (a.k.a. CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2)
  • Tags / Fusion Proteins
    • 6XHis (N terminal on backbone)
    • Smt3 (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    DNASU HsCD00078344

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expressed in a soluble state, except when grown in minimal media to produce 15N labeled protein. Can be purified using N-terminal 6xHis tag and SUMO tag can then be efficiently removed by ULP-1 cleavage yielding the N-terminal methionine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PE-SUMO CRYAB was a gift from Larry David (Addgene plasmid # 217670 ; http://n2t.net/addgene:217670 ; RRID:Addgene_217670)
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