PE-SUMO CRYAB
(Plasmid #217670)

• Purpose: For expression of SUMO tagged human alpha B crystallin
• Depositing Lab: Larry David
• Publication: Completion of previous SUMO tagged human crystallin submission plus plasmids for native chemical ligation to produced gamma S crystallin (unpublished)

Backbone

• Vector backbone: PE-SUMO
• Backbone manufacturer: LifeSensors
• Backbone size w/o insert (bp): 5761
• Total vector size (bp): 6263
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRYAB
• Species: H. sapiens (human)
• Insert Size (bp): 525
• GenBank ID: CRYAB
• Entrez Gene: CRYAB (a.k.a. CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2)
• Tags / Fusion Proteins:
  • 6XHis (N terminal on backbone)
  • Smt3 (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pE-SUMO CRYAB.gbk
• A portion of this plasmid was derived from a plasmid made by: DNASU HsCD00078344

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expressed in a soluble state, except when grown in minimal media to produce 15N labeled protein. Can be purified using N-terminal 6xHis tag and SUMO tag can then be efficiently removed by ULP-1 cleavage yielding the N-terminal methionine.