pET28CLY8
(Plasmid
#21767)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5200
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsBL21(DE3)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats
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Insert Size (bp)1780
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byECFP and EYFP genes were obtained from BD Biosciences
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28CLY8 was a gift from Maarten Merkx (Addgene plasmid # 21767 ; http://n2t.net/addgene:21767 ; RRID:Addgene_21767) -
For your References section:
Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers. Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M. Biochemistry. 2006 Nov 7. 45(44):13183-92. 10.1021/bi061288t PubMed 17073440