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Hygro-P2A-HA-FKBP-WAPL
(Plasmid #217666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1
  • Backbone size w/o insert (bp) 3913
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WapI
  • Alt name
    Repair template for N-terminal tagging of WapI with FKBP36V protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2987
  • Entrez Gene
    WAPL (a.k.a. FOE, KIAA0261, WAPAL)
  • Promoter Lac
  • Tag / Fusion Protein
    • dTag (FKBP36V protein) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (destroyed during cloning)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hygro-P2A-HA-FKBP-WAPL was a gift from Daniel Gerlich (Addgene plasmid # 217666 ; http://n2t.net/addgene:217666 ; RRID:Addgene_217666)

  • For your References section:

    Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase. Batty P, Langer CC, Takacs Z, Tang W, Blaukopf C, Peters JM, Gerlich DW. EMBO J. 2023 Aug 15;42(16):e113475. doi: 10.15252/embj.2023113475. Epub 2023 Jun 26. 10.15252/embj.2023113475 PubMed 37357575
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