Hygro-P2A-HA-FKBP-WAPL
(Plasmid #217666)

• Purpose: Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP
• Depositing Lab: Daniel Gerlich
• Publication: Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi: 10.15252/embj.2023113475. Epub 2023 Jun 26.

Backbone

• Vector backbone: pCR2.1
• Backbone size w/o insert (bp): 3913
• Total vector size (bp): 6900
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WapI
• Alt name: Repair template for N-terminal tagging of WapI with FKBP36V protein
• Species: H. sapiens (human)
• Insert Size (bp): 2987
• Entrez Gene: WAPL (a.k.a. FOE, KIAA0261, WAPAL)
• Promoter: Lac
• Tag / Fusion Protein:
  • dTag (FKBP36V protein) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (destroyed during cloning)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry