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Addgene

RepairTemplate_AAVS1-OsTIR1-F74G-SNAP
(Plasmid #217665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #129719
  • Backbone size w/o insert (bp) 3438
  • Total vector size (bp) 9424
  • Modifications to backbone
    The plasmid was digested with HpaI and AflII; purified the fragment containing the HA-L & HA-R. Blunt-end clone OsTIR1-F74G-SNAP-Blast insert into the backbone
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OsTir1 F74G-SNAP-Blast-AAVS1
  • Species
    O. sativa
  • Insert Size (bp)
    5986
  • Tag / Fusion Protein
    • SNAP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RepairTemplate_AAVS1-OsTIR1-F74G-SNAP was a gift from Daniel Gerlich (Addgene plasmid # 217665 ; http://n2t.net/addgene:217665 ; RRID:Addgene_217665)

  • For your References section:

    Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase. Batty P, Langer CC, Takacs Z, Tang W, Blaukopf C, Peters JM, Gerlich DW. EMBO J. 2023 Aug 15;42(16):e113475. doi: 10.15252/embj.2023113475. Epub 2023 Jun 26. 10.15252/embj.2023113475 PubMed 37357575
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