RepairTemplate_NIPBL_mEGFP_mAID
(Plasmid #217664)

• Purpose: Repair template to endogenously tag NIPBL with mEGFP-mAID Tag
• Depositing Lab: Daniel Gerlich
• Publication: Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi: 10.15252/embj.2023113475. Epub 2023 Jun 26.

Backbone

• Vector backbone: pCR2.1
• Backbone size w/o insert (bp): 3913
• Total vector size (bp): 6327
• Modifications to backbone: The backbone was digested with EcoRV. Insert ordered as gBlock and blunt-end cloned into vector
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: meGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NIPBL_mEGFP_mAID
• Species: H. sapiens (human)
• Insert Size (bp): 2414
• Entrez Gene: NIPBL (a.k.a. CDLS, CDLS1, IDN3, IDN3-B, Scc2)
• Tags / Fusion Proteins:
  • meGFP
  • mAID

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: CAGGAAACAGCTATGAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry