pDEST17-LAP3
(Plasmid
#217661)
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PurposeFor protein purification from Ecoli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST17
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAP3
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SpeciesH. sapiens (human)
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Entrez GeneLAP3 (a.k.a. HEL-S-106, LAP, LAPEP, PEPS)
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7fw (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-4644671/v1 for ResearchSquare preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-LAP3 was a gift from Karla Feijs (Addgene plasmid # 217661 ; http://n2t.net/addgene:217661 ; RRID:Addgene_217661) -
For your References section:
Family-wide analysis of human macrodomains reveals novel activities. Weixler L, Žaja R, Ikenga NJ, Mohan G, Aydin G, Wijngaarden S, Filippov DV, Lüscher B, Feijs-Žaja KLH. ResearchSquare. 2024 Aug 8. doi: 10.21203/rs.3.rs-4644671/v1 10.21203/rs.3.rs-4644671/v1