pCMV_nLightG
(Plasmid
#217656)
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PurposeExpresses norepinephrine indicator nLightG under mammalian promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 3974
- Total vector size (bp) 5932
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenLightG
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SpeciesSynthetic
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Insert Size (bp)1950
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV_nLightG was a gift from Tommaso Patriarchi (Addgene plasmid # 217656 ; http://n2t.net/addgene:217656 ; RRID:Addgene_217656) -
For your References section:
Sensitive multicolor indicators for monitoring norepinephrine in vivo. Kagiampaki Z, Rohner V, Kiss C, Curreli S, Dieter A, Wilhelm M, Harada M, Duss SN, Dernic J, Bhat MA, Zhou X, Ravotto L, Ziebarth T, Wasielewski LM, Sonmez L, Benke D, Weber B, Bohacek J, Reiner A, Wiegert JS, Fellin T, Patriarchi T. Nat Methods. 2023 Sep;20(9):1426-1436. doi: 10.1038/s41592-023-01959-z. 10.1038/s41592-023-01959-z PubMed 37474807