pET28CLY6
(Plasmid #21765)

• Depositing Lab: Maarten Merkx
• Publication: Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-92.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats
• Insert Size (bp): 1750
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ECFP and EYFP genes were obtained from BD Biosciences
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry