Skip to main content
Addgene

pCM4.4
(Plasmid #217648)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCM2
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon Optimized SpCas9
  • Insert Size (bp)
    4107

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM4.4 was a gift from Huimin Zhao (Addgene plasmid # 217648 ; http://n2t.net/addgene:217648 ; RRID:Addgene_217648)

  • For your References section:

    Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces. Ye S, Enghiad B, Zhao H, Takano E. J Ind Microbiol Biotechnol. 2020 May;47(4-5):413-423. doi: 10.1007/s10295-020-02277-5. Epub 2020 May 4. 10.1007/s10295-020-02277-5 PubMed 32367443
Related items: