Drosophila Wdr20-V5
(Plasmid
#217622)
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PurposeMammalian expression of V5 tagged Drosophila Wdr20
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWDR20
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SpeciesD. melanogaster (fly)
Cloning Information
- Cloning method Gene Synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Drosophila Wdr20-V5 was a gift from Ethan Lee (Addgene plasmid # 217622 ; http://n2t.net/addgene:217622 ; RRID:Addgene_217622) -
For your References section:
The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6. Spencer ZT, Ng VH, Benchabane H, Siddiqui GS, Duwadi D, Maines B, Bryant JM, Schwarzkopf A, Yuan K, Kassel SN, Mishra A, Pimentel A, Lebensohn AM, Rohatgi R, Gerber SA, Robbins DJ, Lee E, Ahmed Y. Nat Commun. 2023 Oct 5;14(1):6174. doi: 10.1038/s41467-023-41843-0. 10.1038/s41467-023-41843-0 PubMed 37798281