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Addgene

Drosophila Usp46-V5
(Plasmid #217620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    USP46
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Usp12-46 (a.k.a. Dmel_CG7023, CG7023, Dmel\CG7023, Q9VCT9)

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Drosophila Usp46-V5 was a gift from Ethan Lee (Addgene plasmid # 217620 ; http://n2t.net/addgene:217620 ; RRID:Addgene_217620)

  • For your References section:

    The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6. Spencer ZT, Ng VH, Benchabane H, Siddiqui GS, Duwadi D, Maines B, Bryant JM, Schwarzkopf A, Yuan K, Kassel SN, Mishra A, Pimentel A, Lebensohn AM, Rohatgi R, Gerber SA, Robbins DJ, Lee E, Ahmed Y. Nat Commun. 2023 Oct 5;14(1):6174. doi: 10.1038/s41467-023-41843-0. 10.1038/s41467-023-41843-0 PubMed 37798281
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