pGEX-4T1-GST-TEV-NAP1
(Plasmid
#217610)
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PurposeFor expression in E. coli of NAP1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217610 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T1
- Total vector size (bp) 6000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNAP1
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SpeciesH. sapiens (human)
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Entrez GeneAZI2 (a.k.a. AZ2, NAP1, TILP)
- Promoter TAC promoter
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GEX5 Fw
- 3′ sequencing primer GEX3 Rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T1-GST-TEV-NAP1 was a gift from Sascha Martens (Addgene plasmid # 217610 ; http://n2t.net/addgene:217610 ; RRID:Addgene_217610) -
For your References section:
Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S. bioRxiv September 25, 202 10.1101/2023.09.25.559255