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Addgene

pET28CLY1
(Plasmid #21760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECFP -EYFP linked by flexible one SGGSGG repeats
  • Insert Size (bp)
    1650
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ECFP and EYFP genes were obtained from BD Biosciences

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28CLY1 was a gift from Maarten Merkx (Addgene plasmid # 21760 ; http://n2t.net/addgene:21760 ; RRID:Addgene_21760)

  • For your References section:

    Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers. Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M. Biochemistry. 2006 Nov 7. 45(44):13183-92. 10.1021/bi061288t PubMed 17073440
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