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pDEST27-GST-ATXN1 FL(92Q)-S776D
(Plasmid #21757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST27
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 8123
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B or similar
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ataxin-1
  • Alt name
    ATXN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2604
  • Mutation
    insert contains a stretch of 92 Q(Glutamines) and no stop codon; Serine 776 changed to Aspartic Acidthis is the mutant (expanded) version of Ataxin-1
  • GenBank ID
    NM_000332
  • Entrez Gene
    ATXN1 (a.k.a. ATX1, D6S504E, SCA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer M13 (-21) Forward or T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST27-GST-ATXN1 FL(92Q)-S776D was a gift from Huda Zoghbi (Addgene plasmid # 21757 ; http://n2t.net/addgene:21757 ; RRID:Addgene_21757)

  • For your References section:

    Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1. Lim J, Crespo-Barreto J, Jafar-Nejad P, Bowman AB, Richman R, Hill DE, Orr HT, Zoghbi HY. Nature. 2008 Apr 10. 452(7188):713-8. 10.1038/nature06731 PubMed 18337722
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