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pIVEX2.3-AC17-5c-frGFP
(Plasmid #217525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVEX2.3
  • Total vector size (bp) 4646
  • Vector type
    In vitro transcription-translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AC17-5c riboswitch-regulated folding reporter GFP
  • Species
    Synthetic
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGCGTCCGGCGTAGAGGATCGAGA
  • 3′ sequencing primer AGGGGTTATGCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVEX2.3-AC17-5c-frGFP was a gift from Keisuke Fukunaga (Addgene plasmid # 217525 ; http://n2t.net/addgene:217525 ; RRID:Addgene_217525)

  • For your References section:

    Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches. Ishii Y, Fukunaga K, Cooney A, Yokobayashi Y, Matsuura T. Chem. Commun., 2024 10.1039/D4CC00965G
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