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Addgene

Pepper-fused sgRNA
(Plasmid #217520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone size w/o insert (bp) 2269
  • Total vector size (bp) 2341
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    No other marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    No additional growing instructions
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pepper-fused sgRNA for spCas9
  • gRNA/shRNA sequence
    An empty gRNA expression vector, used to create a gRNA to a specific sequence.
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer caccgggtcttcgagaagacctgtttGagagctaGGCCG
  • 3′ sequencing primer ACaggtcttctcgaagacccggtgtttcgtcctttccac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pepper-fused sgRNA was a gift from Xing Li (Addgene plasmid # 217520 ; http://n2t.net/addgene:217520 ; RRID:Addgene_217520)

  • For your References section:

    Fluorogenic CRISPR for genomic DNA imaging. Zhang Z, Rong X, Xie T, Li Z, Song H, Zhen S, Wang H, Wu J, Jaffrey SR, Li X. Nat Commun. 2024 Jan 31;15(1):934. doi: 10.1038/s41467-024-45163-9. 10.1038/s41467-024-45163-9 PubMed 38296979
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