pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
(Plasmid
#217509)
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PurposeKD Recombinase dependent conditional 1X ALFA cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScriptII KS(-)
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Backbone manufacturerAgilent
- Total vector size (bp) 4327
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
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Alt nameKDRT dependent conditional 1XALFA tag
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1850
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Tag
/ Fusion Protein
- ALFA tag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA was a gift from S. Lawrence Zipursky (Addgene plasmid # 217509 ; http://n2t.net/addgene:217509 ; RRID:Addgene_217509) -
For your References section:
Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, Wohlschlegel JA, Aso Y, Zipursky SL. Neuron. 2024 Jan 13:S0896-6273(23)00973-X. doi: 10.1016/j.neuron.2023.12.014. 10.1016/j.neuron.2023.12.014 PubMed 38262414