Rb379-928/C706F-pGex2TK
(Plasmid #21750)

• Depositing Lab: William Kaelin
• Publication: Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Backbone

• Vector backbone: pGex2TK
• Backbone manufacturer: Pharmacia
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha BL21
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rb
• Alt name: retinoblastoma protein
• Species: H. sapiens (human)
• Insert Size (bp): 1670
• Mutation: deletion mutant ORF contains aa 379-928 of wt Rb cDNA, contains silent mutation at codon 420 (full-length Rb aa R798 (CGG to CGA) introducing an additional EcoR I-site), contains mutation at codon 328 (full-length Rb aa C706F (TCG to TTG) corresponding to a naturally occurring,loss-of-function RB point mutation known to inactivate pocket binding function [Kaye et al., 1990; Kaelin et al., 1991)
• GenBank ID: BC040540
• Entrez Gene: RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: pGex-5'
• 3′ sequencing primer: pGex-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry