LifeAct-MTED
(Plasmid
#217498)
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PurposeExpresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTet-On
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Backbone manufacturerDavid Root, RRID: Addgene_41393
- Backbone size w/o insert (bp) 4621
- Total vector size (bp) 5461
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLifeAct-mScarlet-MTED
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Alt nameLifeAct-MTED
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Alt nameMicrotubule Elimination Domain of Efa6 protein
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SpeciesD. melanogaster (fly)
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Insert Size (bp)840
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LifeAct-MTED was a gift from Erik Dent (Addgene plasmid # 217498 ; http://n2t.net/addgene:217498 ; RRID:Addgene_217498) -
For your References section:
A methodology for specific disruption of microtubule polymerization into dendritic spines. Holland ED, Miller HL, Millette MM, Taylor RJ, Drucker GL, Dent EW. Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi: 10.1091/mbc.E24-02-0093. Epub 2024 Apr 17. 10.1091/mbc.E24-02-0093 PubMed 38630519