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Addgene

Rb-379-928-pGex2TK Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme(s): BamHI.

We expected the following band sizes for this digest: 4.9kb and 1.7kb. We see the expected pattern (assuming the uppermost band in the digest lane is single cut plasmid, which should run at about 6.7kb.