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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS
(Plasmid #217479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5415
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nier1b
  • Species
    Synthetic
  • Insert Size (bp)
    2427
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xNLS, P2A-EGFP-3xNLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS was a gift from Peng Zou (Addgene plasmid # 217479 ; http://n2t.net/addgene:217479 ; RRID:Addgene_217479)

  • For your References section:

    Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators. Lin C, Liu L, Zou P. Cell Rep Methods. 2023 Jul 27;3(8):100544. doi: 10.1016/j.crmeth.2023.100544. eCollection 2023 Aug 28. 10.1016/j.crmeth.2023.100544 PubMed 37671014
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