pcDNA3.4_StrepII_TEV_PP2Ac
(Plasmid
#217477)
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PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePP2Ac
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SpeciesH. sapiens (human)
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Insert Size (bp)981
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GenBank IDX12646.1
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Tag
/ Fusion Protein
- StrepII, TEV (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.4_StrepII_TEV_PP2Ac was a gift from Wolfgang Peti (Addgene plasmid # 217477 ; http://n2t.net/addgene:217477 ; RRID:Addgene_217477) -
For your References section:
Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19. Padi SKR, Vos MR, Godek RJ, Fuller JR, Kruse T, Hein JB, Nilsson J, Kelker MS, Page R, Peti W. Nature. 2024 Jan;625(7993):195-203. doi: 10.1038/s41586-023-06870-3. Epub 2023 Dec 20. 10.1038/s41586-023-06870-3 PubMed 38123684