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myc-mTOR (1-1482 aa)
(Plasmid #21745)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    BD Pharmingen
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTOR (1-1482)
  • Alt name
    RAFT1
  • Alt name
    Frap1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4446
  • Mutation
    amino acids 1-1482
  • Entrez Gene
    Mtor (a.k.a. Frap1, RAFT1)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc-mTOR (1-1482 aa) was a gift from David Sabatini (Addgene plasmid # 21745 ; http://n2t.net/addgene:21745 ; RRID:Addgene_21745)

  • For your References section:

    GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim DH, Sarbassov DD, Ali SM, Latek RR, Guntur KV, Erdjument-Bromage H, Tempst P, Sabatini DM. Mol Cell 2003 Apr;11(4):895-904. 10.1016/S1097-2765(03)00114-X PubMed 12718876
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