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Addgene

LCV2 IRF3 KO
(Plasmid #217446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPRv2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting IRF3
  • Alt name
    IRF3
  • gRNA/shRNA sequence
    CGTGCGGCTCTTGTTCACCC
  • Species
    H. sapiens (human)
  • Entrez Gene
    IRF3 (a.k.a. IIAE7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LCV2 IRF3 KO was a gift from Jarrod Johnson (Addgene plasmid # 217446 ; http://n2t.net/addgene:217446 ; RRID:Addgene_217446)

  • For your References section:

    Cell-free assays reveal that the HIV-1 capsid protects reverse transcripts from cGAS immune sensing. Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI, Johnson JS. PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 10.1371/journal.ppat.1012206. 10.1371/journal.ppat.1012206 PubMed 39874383
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