pJWZ6
(Plasmid #21744)

• Depositing Lab: David Sherwood
• Publication: Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Backbone

• Vector backbone: pBS Sk-
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2931
• Vector type: bacterial propagation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry::dMoeABD
• Alt name: drosophila moesin actin binding domain
• Species: D. melanogaster (fly)
• Insert Size (bp): 1497
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7, M13fwd
• 3′ sequencing primer: T7, M13Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dan Kiehart Duke University
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry