pAA173
(Plasmid #21743)

• Depositing Lab: David Sherwood
• Publication: Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Backbone

• Vector backbone: pBS Sk-
• Backbone manufacturer: stratagene
• Backbone size w/o insert (bp): 5712
• Vector type: bacterial propagation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pie-1>mCherry::PH domain (PLC-delta)
• Species: C. elegans (nematode)
• Insert Size (bp): 10871
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Anjon Audhya University of Wisconsin, Madison
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry