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  • Single-Cell Quantitative Expression Reporters (scQers) Libraries

Single-Cell Quantitative Expression Reporters (scQers) Libraries
(Pooled Libraries #194097, #194098, #217420, #217421, #217422, #217423, #217424, #1000000239)

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Item Catalog # Description Quantity Price (USD)
Pooled Library 194097 pre-associated oBC-mBC backbone (p025) 1 $1300 Add to Cart
Pooled Library 194098 pre-associated oBC-minP-GFP-mBC backbone (p043) 1 $1300 Add to Cart
Pooled Library 217420 scQers promoter control #1 (low: minimal promoter, p027) 1 $380 Add to Cart
Pooled Library 217421 scQers promoter control #2 (low: no promoter, p029) 1 $380 Add to Cart
Pooled Library 217422 scQers promoter control #3 (intermediate: UBC promoter, p041) 1 $380 Add to Cart
Pooled Library 217423 scQers promoter control #4 (intermediate: Pgk1 promoter, p042) 1 $380 Add to Cart
Pooled Library 217424 scQers promoter control #5 (high: EEF1A1 promoter, p028) 1 $380 Add to Cart
Pooled Library 1000000239 scQers promoter control series (p027, p028, p029, p041, p042) 1 $1300 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Plasmids

    p025 and p043: ~1,200,000
  • Control Plasmids

    • p027: 250
    • p029: 250
    • p041: 250
    • p042: 250
    • p028: 250

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    20 ng/µL

Resource Information

Depositor Comments

The data and code (Link opens in a new window) related to these libraries is available on GitHub.

Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519236v1?rss=1 (Link opens in a new window) for bioRxiv preprint.

A schematic diagram shows cassette with, the left most “detection” section containing a promoter and DNA segment labelled, “oBC” with an arrow leading to “oRNA” below. The right most “quantification” section contains a DNA segment labelled, “mBC”. Between these two sections is a DNA segment labeled "CRE", a promoter, and another DNA segment. Another arrow leads from the end of the unlabeled DNA segment and the quantification section to “mRNA” that contains the mBC. Below is a schematic of a device leading to a pool of cells. Dotted lines labeled “absent” lead to three empty cells with a dotted line leading below to the text “mBC=ND”. Two arrows with check marks also lead down from the pool of cells. The left one leads to a cell with plasmids in the cytoplasm and a further arrow leads down to the text, “mBC=0”. mBC=ND and mbc=0 can be detected via oBC. The right arrow has a miniaturized schematic of linear DNA from above, labeled “reporter #1?” and leads to a cell with plasmids and mRNAs in the cytoplasm labeled “scRNA-seq”, and a further arrow leads down to the text “mBC=7” and can be quantified via mBC.

Figure 1: Schematic of cassette and workflow for single-cell quantitative expression reporters (scQers). Single-cell quantitative expression reporters (scQers) are dual barcoded RNA cassettes that separate the detection and quantification tasks, providing scalable and high-sensitivity readout of reporter expression in pooled single-cell contexts. Upon integration of libraries in multicellular system and single-cell profiling, the constitutively expressed detection barcode (blue, oBC) discriminates which cell received which reporter, and the matched quantification barcode (red, mBC) then enables unbiased measurement of the mRNA production from the associated cis-regulatory element.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Single-cell quantitative expression reporters (scQers) pre-associated oBC-mBC backbone (p025) Libray was a gift from Jay Shendure (Addgene #194097)
    Single-cell quantitative expression reporters (scQers) pre-associated oBC-minP-GFP-mBC backbone (p043) Libray was a gift from Jay Shendure (Addgene #194098)
    Single-cell quantitative expression reporters (scQers) promoter control #1 (low: minimal promoter, p027) Libray was a gift from Jay Shendure (Addgene #217420)
    Single-cell quantitative expression reporters (scQers) promoter control #2 (low: no promoter, p029) Libray was a gift from Jay Shendure (Addgene #217421)
    Single-cell quantitative expression reporters (scQers) promoter control #3 (intermediate: UBC promoter, p041) Libray was a gift from Jay Shendure (Addgene #217422)
    Single-cell quantitative expression reporters (scQers) promoter control #4 (intermediate: Pgk1 promoter, p042) Libray was a gift from Jay Shendure (Addgene #217423)
    Single-cell quantitative expression reporters (scQers) promoter control #5 (high: EEF1A1 promoter, p028) Libray was a gift from Jay Shendure (Addgene #217424)
    Single-cell quantitative expression reporters (scQers) control series (p027, p028, p029, p041, p042) Libraries were a gift from Jay Shendure (Addgene #1000000239)

  • For your References section:

    Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters. Lalanne JB, Regalado SG, Domcke S, Calderon D, Martin B, Li T, Suiter CC, Lee C, Trapnell C, Shendure J. bioRxiv 2022.12.10.519236. doi: 10.1101/2022.12.10.519236.
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