pRetroNeo
(Plasmid
#21740)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Modifications to backboneN/A
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Vector typeN/A
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsAlso resistant to Kanamycin. Pir1 cells (Invitrogen)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byClare Huxley
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRetroNeo was a gift from Clare Huxley (Addgene plasmid # 21740 ; http://n2t.net/addgene:21740 ; RRID:Addgene_21740) -
For your References section:
Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 - new vectors for in vitro and in vivo delivery. Magin-Lachmann C, Kotzamanis G, D'Aiuto L, Wagner E, Huxley C. BMC Biotechnol. 2003 Feb 3. 3(1):2. 10.1186/1472-6750-3-2 PubMed 12609052