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Addgene

pRetroNeo
(Plasmid #21740)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21740 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    N/A
  • Modifications to backbone
    N/A
  • Vector type
    N/A
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    Also resistant to Kanamycin. Pir1 cells (Invitrogen)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Clare Huxley

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroNeo was a gift from Clare Huxley (Addgene plasmid # 21740 ; http://n2t.net/addgene:21740 ; RRID:Addgene_21740)
  • For your References section:

    Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 - new vectors for in vitro and in vivo delivery. Magin-Lachmann C, Kotzamanis G, D'Aiuto L, Wagner E, Huxley C. BMC Biotechnol. 2003 Feb 3. 3(1):2. 10.1186/1472-6750-3-2 PubMed 12609052