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Addgene

pSM045
(Plasmid #217399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBTrcK
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5403
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BTE1
  • Alt name
    BTE
  • Species
    Synthetic
  • Insert Size (bp)
    906
  • Promoter PTrc

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGAAACAGACCATTAGGAGG
  • 3′ sequencing primer TCTCTCATCCGCCAAAACAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSM045 was a gift from Brian Pfleger (Addgene plasmid # 217399 ; http://n2t.net/addgene:217399 ; RRID:Addgene_217399)

  • For your References section:

    Expanding the synthetic biology toolbox of Cupriavidus necator for establishing fatty acid production. Mishra S, Perkovich PM, Mitchell WP, Venkataraman M, Pfleger B. J Ind Microbiol Biotechnol. 2024 Feb 16:kuae008. doi: 10.1093/jimb/kuae008. 10.1093/jimb/kuae008 PubMed 38366943
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